Kindiki said leaders should not involve the youth in activities that could threaten peace and safety. In remarks that have attracted attention, he warned those involved that their political days could be coming to an end.
The latest exchange comes as political activity continues to increase across the country ahead of the 2027 General Election.
Gachagua, who now leads the Democracy for Citizens Party (DCP), has been openly positioning himself against President William Ruto and building support in different parts of the country.
The relationship between Gachagua and Kindiki has remained tense since Gachagua was removed from the Deputy President's office in 2024.
Kindiki later took over the position and has since emerged as one of the key figures in the Kenya Kwanza administration.
The two leaders have also differed over the role of young people in politics. Gachagua has previously spoken about the need to protect young Kenyans and accused the government of failing to address insecurity and political violence. Recent remarks from both camps have further increased the political debate.
Kindiki's latest warning comes at a time when politicians are increasingly turning to young people to mobilise supporters as the 2027 election approaches.
The Deputy President has previously attacked Gachagua's political agenda, while Gachagua has continued to criticise the Ruto administration.
In July, Gachagua even asked his supporters to stop attacking Kindiki and instead focus their criticism on President Ruto and Interior CS Kipchumba Murkomen.
The latest confrontation shows that the political battle between the two camps is far from over.
With less than a year remaining before the 2027 election, their growing exchanges could further shape the political contest, especially in the Mt Kenya region.
For now, Kindiki's warning has added another layer to the increasingly heated political debate surrounding Gachagua and the government.